Generator

Part:BBa_K814008

Designed by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn   Group: iGEM12_Minnesota   (2012-10-03)

ScyB (leucine dehydrogenase) generator

Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB (NpR1275), an acetolactate synthetase, carries out the first step in scytonemin biosynthesis, converting L-tryptophan to 3-indole pyruvic acid.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyB. This part can be used to express ScyB protein in E. coli.

Balskus and Walsh, 2009. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766776/] Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 849
    Illegal NotI site found at 1202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 849
    Illegal BglII site found at 134
    Illegal XhoI site found at 1210
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


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